Next Generation Fully Integrated Digital Assay Design
Unlike other digital assay technology BLINK Beads integrate sample preparation with a complete analysis workflow. By using the same carrier for nucleic acid extraction/enrichment, extraction yield is improved, and total captured material is used in the analysis, providing more precise and sensitive results.
Nucleic acid extraction steps and loading of amplification reagents are performed on the Processing Rack.
A wash step removes un-specifically bound materials leaving purified nucleic acid bound to the Bead.
Beads are separated by oil and contain defined volume of amplification solution.
Beads are placed in wells and aligned in monolayer formation for thermocycling and fluorescence imaging.
PCR amplification and fluorescence detection are performed on the Beads with the BLINK X Instrument.
Using BLINK Beads you can apply the principles of digital assay design beyond PCR to create any class of assay.
Digital assays are based on partitioning the sample into thousands of independent reactions, a function that the BLINK Beads are optimally designed to achieve. After being loaded with targets and amplification reagents and transferred to oil, each Bead forms an isolated reaction compartment for performing independent enzymatic reactions. This approach is typically utilised for PCR based assays; the Beads provide the technology to apply this principle to other enzymatic assays.
BLINK has accelerated digital PCR to the next generation.
BLINK Beads provide microfluidics-free digital PCR, enabling unprecedented performance and novel assay schemes to empower your next scientific breakthrough.
With the ability to provide a complete analysis workflow on a single reagent carrier, single molecule sensitivity, exquisite quantification and results in 15 minutes the BLINK Beads coupled with the BLINK X platform are the pathway to endless possibilities.
BLINK’s design drastically reduces time to result.
By integrating sample prep and the entire assay workflow within individual Beads workflows are completed in 15 minutes from sample to result.
All material is amplified in a minimised volume distributed across thousands of individual compartments which provide for optimal thermal control, enabling fast cycling on BLINK’s hardware.
Microfluidics-free absolute target quantitation with pre-made Bead nanoreactors. Single molecule sensitivity with wide measurement range.
Ultraplexing is highly flexible and sensitive multiplexing. It provides fully quantitative detection for each target across the entire measurement range without cross-interference from other targets in the panel.
Selective encoding of individual samples with encoded Beads, allowing for parallel processing of multiple samples in one test assay.
While our development of the BLINK Beads has revealed many of their capabilities, there is still much to be discovered.
Register your interest in the BLINK X today.