Primers and Probes are reversibly bound to the encoded Beads so that different Beads can be used for sample prep. Once accommodated in oil, the primers and probes are released for amplification and detection. Since each reaction is carried out in an individual compartment, Beads, and as such assays, with different target specificities can be safely combined in a single workflow without cross interference.
Melting curve analysis using intercalating dyes or molecular beacons on BLINK nanoreactor Beads allows for easy SNP detection and quantitation of SNPs or other genetic variants in a digital format.
BLINK Beads can be customized for specific applications with different binding specificities, primers and probes.
BLINK Beads comprise a hydrogel matrix that defines the actual space for amplification and detection. Each Bead is encoded with a fluorescent code and carries magnetic particles for ease of handling. Moreover, the matrix is derivatized with streptavidin providing a simple means for reversibly attaching primer and probe oligonucleotides to the matrix. The hydrogel matrix is coated with a crosslinked polymer for efficient binding of nucleic acids.
In a suitable binding buffer nucleic acids contained in a sample bind to the Bead surface.
A wash step removes un-specifically bound materials leaving purified nucleic acid bound to the Bead.
Beads are loaded with generic amplification & detection reagents (enzymes, buffers, dNTPs).
Beads are suspended in oil
Hydrogel core liquifies at elevated temperature and forms a droplet. Primers and probes are released. Enzymes are activated. PCR begins. All code and magnetic particles are confined within a space formed by the contracting binding layer, thus clearing up the nanoreactor space for amplification and fluorescence detection.
Target specific amplification is performed within the nanoreactor space formed by the Bead.
Due to spatial separation fluorescence signal formed by the amplification reaction can be read simultaneous with the fluorescent Bead code by fluorescence imaging.
Currently BLINK’s technologies and products are accessible through strategic collaborations with BLINK as a development partner in selected application areas. Test developers and researchers will be able to develop their assays and applications independently on the BLINK X platform utilising validated components while maintaining design flexibility.